脐带血(UCB)富含hematopoetic干细胞(hsc)。我们有孤立的小说细胞系人口干细胞自我更新的人类的联合展览性质,但没有在肝星状细胞表面标记,通常是发现。记录分析表明这些细胞转录因子表达,Oct-4 Rex-1, Sox-2通常表达的干细胞。我们称这些小说细胞为nonhematopoietic脐带血干细胞(nh-UCBSCs)。先前的研究已经表明,静脉输液UCBCs可以改善神经赤字引起的缺血性脑损伤。细胞的身份调解这恢复的效果,然而,尚未确定。我们假定nh-UCBSCs可能的来源的联合细胞能够调节这些影响。为了验证这个假设,我们静脉注射一百万个人类nh-UCBSCs注入大鼠短暂单侧大脑中动脉闭塞后48 h。其它实验动物组注射了生理盐水注射或RN33b神经干细胞。动物神经功能检测nh-UCBSCs注入之前,后来在不同时间段使用电池的行为测试。 In limb placement tests, animals treated with nh-UCBSCs exhibited mean scores that were significantly better than animals treated with RN33b neural stem cells or saline. Similarly, in stepping tests, nh-UCBSC-treated animals again exhibited significantly better performance than the other experimental groups of animals. Analysis of infarct volume revealed that ischemic animals treated with nh-UCBSCs exhibited a 50% reduction in lesion volume in comparison to saline-treated controls. Histological analysis of brain tissue further revealed the presence of cells that stained for human nuclei. Some human nuclei-positive cells were also co-labeled for NeuN, indicating that the transplanted cells expressed markers of a neuronal phenotype. Cells expressing the human nuclei marker within the brain, however, were rather scant, suggesting that the restorative effects of nh-UCBSCs may be mediated by mechanisms other than cell replacement. To test this hypothesis, nh-UCBSCs were directly transplanted into the brain parenchyma after ischemic brain injury. Sprouting of nerve fibers from the nondamaged hemisphere into the ischemically damaged side of the brain was assessed by anterograde tracing using biotinylated dextran amine (BDA). Animals with nh-UCBSC transplants exhibited significantly greater densities of BDA-positive cells in the damaged side of the brain compared to animals with intraparenchymal saline injections. These results suggest that restorative effects observed with nh-UCBSC treatment following ischemic brain injury may be mediated by trophic actions that result in the reorganization of host nerve fiber connections within the injured brain.