PT -期刊文章盟Rittika呕吐AU -克里斯托弗Linington AU -弗朗西斯克格劳盟莉迪亚萨瓦特AU -温弗利储料器AU -拉斯科莫罗夫斯基盟-基督教Probst TI -重组细胞间接免疫荧光试验检测IgM副蛋白的多神经病(P5.120) DP - 2017年4月18日TA -神经病学PG - P5.120 VI - 88 IP - 16补充4099 - //www.ez-admanager.com/content/88/16_Supplement/P5.120.short 4100 - //www.ez-admanager.com/content/88/16_Supplement/P5.120.full所以Neurolo首页gy2017 4月18日;88 AB -目的:开发一种敏感和具体试验检测与myelin-associated反应的循环IgM副蛋白糖蛋白(MAG)多神经病患者。背景:这些专门镁活性IgM副蛋白绑定一个N-linked sulfoglucuronyl碳水化合物抗原决定基(HNK-1)也出现在其他神经糖蛋白等P0, N-CAM和鞘糖脂。在这里,我们报告的开发和验证一个间接免疫荧光试验(IFA)抗体的检测。设计/方法:全身人类MAG c端oligohistidine标签,野生型beta 1, 3-glucuronyltransferase 1 (GlcAT-P)、惰性酶GlcAT-P [E284A],和HNK-1 sulfotransferase (HNK-1ST)表示单独或以不同的组合在HEK293细胞中。表达HNK-1抗原决定基随后分析了免疫印迹和IFA生活和固定细胞使用HNK-1特定的鼠单克隆抗体。随后,IFA使用HEK293-GlcAT-P细胞被用来屏幕HNK-1活性在血清IgM anti-MAG阳性病例的多神经病(n = 40), anti-NMDAR脑炎(n = 20)患者和健康献血者(n = 20)。结果:观察HNK-1主题的表达生活和固定HEK293表达GlcAT-P而缺席在HEK293细胞中表达杂志,HNK-1ST和/或GlcAT-P E284A GlcAT-P缺席的。使用HEK293-GlcAT-P HNK-1 IgM反应被IFA检测细胞从所有anti-MAG患者血清IgM多神经病有关,但在所有的控件。所有血清-当测试使用GlcAT-P -细胞系。结论:重组表达GlcAT-P足以使HEK293细胞合成HNK-1抗原决定基并整合HNK-1-bearing糖蛋白或糖脂质膜的外表面。 This strategy provides a simple approach to detect HNK-1 reactive IgM paraproteins with high sensitivity and specificity in patients with anti-MAG associated polyneuropathy.Disclosure: Dr. Chunder has received personal compensation for activities with EUROIMMUN AG as an employee. Dr. Linington has nothing to disclose. Dr. Graus has nothing to disclose. Dr. Sabater has nothing to disclose. Dr. Stöcker has nothing to disclose. Dr. Komorowski has nothing to disclose. Dr. Probst has nothing to disclose.