@article {PerriotS55.008作者= {Sylvain Perriot和含有杏仁的Mathias Guillaume Perriard和马修-卡纳莱斯和尼尔斯·Jonkmans尼古拉•米{\ ' e} rienne和C {} \ ' e cile默和莉娜El Kassar Anselme毕雷矿泉水和大卫Laplaud和Myriam Schluep和妮可·D {\ ' e} glon Renaud Du Pasquier}, title ={人类诱导多能干细胞星形胶质细胞是由多个Sclerosis-associated不同激活细胞因子(S55.008)},体积={92}={15}补充数量,elocation-id = {S55.008} ={2019},出版商= {Wolters Kluwer健康,公司代表美国神经病学学会},文摘={目的:建立最优协议获得休息星形胶质细胞来源于人类诱导多能干细胞(hiPSC)和检查这些细胞如何应对多发性硬化症(MS)的关键细胞因子。首页背景:最近的研究强调了星形胶质细胞在神经炎症疾病的重要性,与其他中枢神经系统细胞密切互动,还与免疫系统。特别是关于女士,小鼠模型表明,星形胶质细胞疾病,可能是至关重要的球员在早期阶段产生一种保护作用,但是在慢性阶段有害的影响。然而,由于很难获得人类的星形胶质细胞,他们在人类疾病中的作用仍差的特点。设计/方法:我们hiPSCs来自血液(成红血球细胞)从三个健康对照组和4 MS患者和没有胎牛血清分化为星形胶质细胞。首先,使用流式细胞仪分析,基因表达(Fluidigm)和转录组(RNA序列),我们评估hiPSC-derived星形胶质细胞的细胞表现出真正的特征进行比较与人类主要的星形胶质细胞。其次,利用RNA序列和Luminex,我们检查这些hiPSC-derived星形胶质细胞对MS-relevant细胞因子的反应。结果:基因表达和功能分析表明,我们的协议持续产生高纯度人口跨13 hiPSC星形胶质细胞分化成熟。使用这个新模型,我们首先强调的重要性产生休息星形胶质细胞星形胶质细胞无血清媒体文化。第二,我们评估病患IL-1β,TNFα和il - 6,所有重要细胞因子在神经炎症,如多发性硬化症。结论:在这里,我们开发了一种新的无血清协议向星形胶质细胞区分人类万能。我们的研究揭示了非常具体的反应性星形胶质细胞根据引发刺激。 This new model provides ideal conditions for in-depth and unbiased characterization of astrocyte reactivity in neuroinflammatory conditions.Disclosure: Dr. Perriot has nothing to disclose. Dr. Mathias has nothing to disclose. Dr. Perriard has nothing to disclose. Dr. Canales has nothing to disclose. Dr. Jonkmans has nothing to disclose. Dr. M{\'e}rienne has nothing to disclose. Dr. Meunier has nothing to disclose. Dr. El Kassar has nothing to disclose. Dr. Perrier has nothing to disclose. Dr. Laplaud has nothing to disclose. Dr. Schluep has nothing to disclose. Dr. D{\'e}glon has nothing to disclose. Dr. Du Pasquier has received personal compensation for consulting, serving on a scientific advisory board, speaking, or other activities with Biogen, Roche, and Sanofi.}, issn = {0028-3878}, URL = {//www.ez-admanager.com/content/92/15_Supplement/S55.008}, eprint = {//www.ez-admanager.com/content}, journal = {Neurology} }